Genetic analysis of Bacillus stearothermophilus by protoplast fusion
نویسندگان
چکیده
منابع مشابه
Genetic analysis of Bacillus stearothermophilus by protoplast fusion.
Efficient and reliable protoplasting, regeneration, and fusion techniques were established for the prototrophic strain Bacillus stearothermophilus NUB36. Auxotrophic mutants were isolated, and protoplast fusion was used to construct isogenic mutant strains and for chromosomal mapping. Markers were mapped using two-, three-, and four-factor crosses. The order of the markers was hom-1-thr-1-his-1...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1986
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.165.3.994-1001.1986